Yellowstone's Microbes

Powerpoint slides

Class of 2004

The Class of 2003
At Nymph creek, we saw healthly biomass of putative Aquificales. The sample we collected and then analyzed by PCR (using 16S rDNA primers specific for the Aquificales, below), we showed that indeed these organisms are members of the order, Aquificales. Since they are growing so well at low pH, they are probably related to the only acidophilic Aquificales isolate, Hydrogenobaculum acidophilum. At "New Pit", Mammoth Terraces, the microbial mats we saw were composed primarily of 2 different anoxygenic phototrophs: the purple sulfur bacterium- Chromatium, a proteobacterium, and the green sulfur bacterium, Chlorobium. Recall that these phototrophs do not evolve oxygen during photosynthesis, but rather produce sulfur as a biproduct of photosynthesis.
Melissa's spagetti pebbles The effects of DEET at Mosquito Cave
Norm using the handheld microscope to observe the gliding motion of the cyanobacterium, Oscillatoria Aquificales filaments at Mammoth Terraces. These are chemolithoautotrophic thermophiles

This is probably a Sulfurihydrogenibium sp.

DNA extraction and PCR at Lamar Oscillatoria
Baby bear.... do not approach! Dave showing us the ROV (remotely operated vehicle) that is being used for studying the biogeochemical and microbial processes in Yellowstone Lake
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